Table 1.
Strains
| Strain | Genotype | Plasmid |
|---|---|---|
| IH3964 | MATα ho∷HO-ADE2 can1∷HO-CAN1 ash1Δ∷TRP1 | None |
| MY158 | MATα ho∷HO-ADE2 can1∷HO-CAN1 ash1Δ∷TRP1 | pMM111 (ASH1) |
| MY159 | MATα ho∷HO-ADE2 can1∷HO-CAN1 ash1Δ∷TRP1 | pMM112 (ASH1C502R) |
| MY160 | MATα ho∷HO-ADE2 can1∷HO-CAN1 ash1Δ∷TRP1 | pRS316 (vector) |
| MY185 | MATα ho∷HO-ADE2 can1∷HO-CAN1 ash1Δ∷TRP1 | pAJ1621 pEG203 (pLexA) |
| MY186 | MATα ho∷HO-ADE2 can1∷HO-CAN1 ash1Δ∷TRP1 | pAJ1621 pMM94 (pLexA-ASH1) |
| MY208 | MATα ho∷HO-ADE2 can1∷HO-CAN1 ash1Δ∷TRP1 | pAJ1621 pMM129 (pLexA-ΔC90) |
| MY209 | MATα ho∷HO-ADE2 can1∷HO-CAN1 ash1Δ∷TRP1 | pAJ1621 pMM131 (pLexA-ΔN300) |
| MY210 | MATα ho∷HO-ADE2 can1∷HO-CAN1 ash1Δ∷TRP1 | pAJ1621 pMM134 (pLexA-ΔC288) |
| MY211 | MATα ho∷HO-ADE2 can1∷HO-CAN1 ash1Δ∷TRP1 | pAJ1621 pMM127 (pLexA-C502R) |
| MY212 | MATα ho∷HO-ADE2 can1∷HO-CAN1 ash1Δ∷TRP1 | pAS174 (2μ ASH1) |
| MY6 | MATα ho | None |
| MY115 | MATα ho ash1Δ∷LEU2 | None |
Strains used were derivatives of W303, whose complete genotype is ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,13 ura3-52 GAL+ psi+, bar1-1.