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. 2010 Sep;20(9):1219–1228. doi: 10.1101/gr.106245.110

Figure 3.

Figure 3.

Genome organization of centromere region of chromosome 1 and chromosome 2. (A) DNAs from BAC clones hybridized with Cen1 were digested with HindIII and characterized by Southern analysis. Various BAC clones containing unique sequences and the 1.8-kb repeat-unit sequence were obtained. (B) A pulsed-field gel electrophoresis (PFGE) analysis was performed to determine the length consisting of the repeat-unit. Information of restriction enzyme sites is shown in C. (C) Genome organization around Cen1 region. Sequences of contigs from BAC002H09 and BAC003G11 are deposited in the DDBJ/EMBL/GenBank database with accession numbers AB556732–AB556734. Sequencing of BAC clones identified the exact boundary between unique region and repeat region. The length consisting of the repeat-unit was determined by a PFGE analysis. (D) DNAs from BAC clones hybridized with Cen2 were digested with HindIII and characterized by Southern analysis. Various BAC clones containing unique sequences and the 3.0-kb repeat-unit sequence were obtained. (E) A PFGE analysis was performed to determine the length consisting of the repeat-unit. Information of restriction enzyme sites is shown in F. (F) Genome organization around Cen2 region. Sequences of contigs from BAC074F14 and BAC285H02 are deposited in the DDBJ/EMBL/GenBank database with accession numbers AB556735 and AB556736, respectively. Sequencing of BAC clones identified the exact boundary between unique region and repeat region. The length consisting of the repeat-unit was determined by a PFGE analysis.