Fig. 4.

siRNA-mediated depletion of endogenous PHP exerts no significant effects on glucose-induced activation of Rac1 in INS 832/13 cells. INS 832/13 cells transfected with either scrambled siRNA or PHP-siRNA were exposed to either low (2.5 mM) or high (20 mM) glucose for 15 min, as indicated in the text. Relative degree of Rac1 activation in these cells was quantitated by PAK-PBD pulldown assay. For the purpose of controls, total Rac1 from lysates was also determined, and the data suggest no significant effects of PHP depletion on total Rac1 content. Data are representative of 2 separate experiments yielding identical results.