Fig. 7.

A modest but significant increase in the expression of PHP in INS 832/13 cells and normal rat islets following chronic exposure to high glucose; evidence for increased expression of PHP in islets from the Zucker diabetic fatty (ZDF) rat. INS-832/13 cells (A) or normal rat islets (C) were incubated in the presence of glucose (5–30 mM) for 24 h, after which PHP levels were determined by Western blot. Actin was used as a loading control. B and D: densitometric ratios of PHP to actin. Data are means ± SE from 3 independent experiments. *P < 0.05 vs. 5 mM glucose. E: PHP levels were determined by lysates from islets of lean control and ZDF rats by immunoblot. Actin was used as a loading control. A representative blot from 2 experiments is shown here. F: densitometric ratios of PHP to actin; data are means ± SD from 2 experiments. *P < 0.05 vs. lean control islets.