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MG preculture decreases Trx-apoptosis-regulating kinase-1 (ASK1) binding (A) and increases p38 MAPK activation (B). A and B, top: representative Western blots. Bar graphs represent density analysis (n = 5–7 independent experiments). Effect of AG treatment on MG enhanced Trx-ASK1 dissociation (C) and p38 MAPK phosphorylation (D) in cardiomyocytes subjected to SI-R. C and D, top: representative Western blots. Bar graphs represent density analysis (n = 5–6 independent experiments).
