Fig. 5.

Dox-induced EGFP expression in other parts of the urinary tract. Double transgenic UPII-rtTA-M2/TRE-EGFP mice were treated with Dox for 10 days and their renal pelvis (A), ureter (B), prostatic urethra (C), and penile urethra (D) were paraffin-sectioned and stained with anti-EGFP (red), anti-keratin 5 (green; as indicated in A), and DAPI (blue). Note that, with the exception of penile urethra which is lined by squamous epithelium, EGFP expression could be induced in other parts of the urinary tract lined by the urothelium. A, inset (top right dashed box) was an enlarged view of renal pelvic urothelium showing both basal and suprabasal EGFP expression. Magnification of all panels: ×200.