Fig. 2.

NCC surface expression after TPA treatment in cells expressing K44A dynamin. mDCT cells were grown to confluence, transfected with hemeagglutinin-tagged with either wild-type (DW) or K44A (DN) dynamin as described, then treated with 100 nM TPA (T) or vehicle (C) for 15 min before labeling with biotin at 4°C. Biotinylated (cell surface) proteins were recovered using streptavidin-agarose and immunoblotted for NCC. NC, no biotin control; DW+T, wild-type dynamin plus TPA; DN+T, DN plus TPA. Accompanying densitometry is shown (n = 4). *P < 0.05.