Table 2.

Androgen concentration in CaP cell culture and prostate tissue and comparison of APPI and ESI results^a

	APPI Analysis							ESI Analysis
Sample	ASD	T	5-ADiol	DHEA	5α-ASD	DHT	AND	ASD	T	5-ADiol	DHEA	5α-ASD	DHT	AND
CWR-R1 Cells, 24 h 0.1 uM 5-androstan-3α,17β-diol treatmentb	0.150	0.836	1.05	0.225	NA	24.0	9.21	0.139	0.828	ND	NQ	5.745	23.7	7.26
CWR-R1 Cells, 48h 0.1 uM 5-androstan-3α,17β-diol treatmentb	0.392	1.84	1.79	0.672	NA	5.90	4.80	0.359	1.78	ND	NQ	2.40	5.98	4.12
PC-3 Cells, 24 h 0.1 uM 5-androstan-3α,17β-diol treatmentb	0.341	0.102	ND	0.284	12.0	5.76	58.5	0.314	0.095	ND	NQ	13.7	6.39	51.6
Androgen Stimulated Rat Prostate #1c	0.22	0.48	ND	3.42	ND	1.24	NQ	0.32	0.48	ND	ND	NQ	2.04	NQ
Androgen Stimulated Rat Prostate #2c	0.80	1.22	ND	4.36	ND	4.44	NQ	1.06	1.42	ND	ND	NQ	4.28	NQ
Recurrent CaP #1c	0.18	0.06	ND	5.28	ND	0.58	1.02	0.24	0.04	ND	ND	NQ	0.70	ND
Recurrent CaP #2c	0.26	0.52	ND	4.64	ND	ND	3.6	0.24	0.54	ND	ND	NQ	0.66	ND
Benign Human Prostate Peripheral Zone #1c	1.18	0.60	40.8	224	ND	1.46	NQ	ND	0.18	ND	ND	NQ	61.2	NQ
Benign Human Prostate Peripheral Zone #2c	1.42	0.72	93.4	204	21.2	1.8	NQ	2.32	0.36	ND	NQ	ND	128	NQ
Benign Human Prostate Transition Zone #1c	2.72	1.48	ND	78.1	ND	9.74	NQ	1.12	5.44	ND	ND	ND	115	NQ
Benign Human Prostate Transition Zone #2c	0.46	0.52	ND	15.1	ND	1.6	NQ	0.16	0.46	ND	NQ	ND	110	NQ

^aNA = not analyzed; ND = not detected; NQ = S:N < 3 or ratio of quantifying ion to confirmatory ion out of accepted range

^bng/mL media

^cng/g tissue