Table 1.
Primer sequences and product sizes for hMel-1B, GPR50, and RORα exons.
| Gene | Position | Forward Primer | Reverse Primer | Size (bp) |
|---|---|---|---|---|
| hMel-1B | Exon 1 | GGGTCAGGGACAAGATCGA | AGCTGGGCAGGGAAGAGAG | 391 |
| hMel-1B | Exon 2 | TGGCAAAGATCACAAACACC | TCTCGTGCTGACTGTTGCTC | 596 |
| hMel-1B | Exon 2 | AGTTTGTCATGCTGCTGGTG | CCTATCGCTGTCGTGTCCTT | 491 |
| GPR50 | Exon 1 | attcggggagctcaaaaact | agagagggaggcacgcttt | 380 |
| GPR50 | Exon 2 | ggtccagatcctcacgtagc | cctttctccctccctcgata | 482 |
| GPR50 | Exon 2 | gcttagggtggccagagg | ttaccatcgtctgcatccac | 594 |
| GPR50 | Exon 2 | gtagtggcaggcttgggata | gaatgtccggaatgttccat | 489 |
| GPR50 | Exon 2 | tgcagtaaggcatctcactttg | ccaagtctgccttcagtgct | 476 |
| RORα | Exon 1 | CACAGTGATCAGCAGAGCAAA | CATTCTTTGTGGCTGGGATT | 376 |
| RORα | Exon 2 | AGGAGGCCTTTGGATTCACT | CAAGGCAGTGGCCAACTAAT | 174 |
| RORα | Exon 3 | GGCTTCCCCTTTCTCTTCAT | GTTCAAGCATTTGGGGATGT | 297 |
| RORα | Exon 4 | AGTGGAGACATACAAATCACAAAGA | TAACTGGTTGCGTGCTTCTG | 186 |
| RORα | Exon 5 | CCCGTGCAACTGTACAACTC | CCACTCATTCTGACACTAACCTG | 228 |
| RORα | Exon 6 | TGAATAGAGCATCCCAGGAGA | CAAAGCTCGCAGGAGAGAAT | 504 |
| RORα | Exon 7 | CCTTCCCAATATTGCCTCAA | TGTGATCTCCCGTTTTCTCC | 196 |
| RORα | Exon 8 | TCAAAATTCACTTGCAAAGCA | GGCATGCTTTGCTTTCATAAC | 247 |
| RORα | Exon 9 | TTGTCCAAGCAGAGCTTTCA | ATGACCCAAATGACCCAAAC | 229 |
| RORα | Exon 10 | CGTATGGGCCTTGTAAAAATTC | AAACTGTCTTTGTTTTCATTTTGG | 211 |
| RORα | Exon 11 | ATAGTTCAGGCCATGCCAAC | CACCATGTCAGAGGAATGCT | 199 |
| RORα | Exon 12 | TCAAGACAGAAAAAGGGTCCA | GGTTCCCTGATCTTTGATGC | 473 |
| RORα | ALT-SPL 1-1 (V11) | CCCCTCTCCAGCCTCCTAC | CTTTTTCCCTCCTGCTTTCG | 351 |
| RORα | ALT SPL 1-2 (V12) | GGTCACAGCAGCCAGACATA | ACTAAACGCCTTCCTGAGCA | 200 |
| RORα | ALT-SPL 2-3 (V32) | GCAGACAGACACTGACATGCT | GGGTTGACGCTGTTCTGTTT | 233 |
| RORα | ALT-SPL 4-1 (V41) | GGACCCAAGAGACTGACAGG | CTCTTATGGCTCGGGATGG | 445 |
*
The positions of the alternative splice sites for RORα are denoted by “ALT-SPL.”