Fig. 6.

Inactivation of IKKβ and accumulation of phosphorylated IKKα in Glrx1^−/− mice in response to CS exposure. A: lung homogenates were subjected to immunoprecipitation with IKKα and IKKβ antibodies, and immunoblotted with streptavidin-conjugated HRP (top) and IKKα and IKKβ (lower) antibodies. B: IKKα and IKKβ immunoprecipitates separated under nonreducing conditions, and then immunoblotted with anti-GSH (top) and IKKα and IKKβ (lower) antibodies. C: immunoprecipitated IKKα and IKKβ were analyzed for phosphorylation of serine residues using specific phospho-serine antibody, respectively. Gel pictures shown are representative of at least 3 separate experiments.