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DUOX2 mRNA expression levels in HBE1 cells after rhinovirus (RV) infection with or without ATRA. HBE1 cells were grown in ALI culture conditions with or without 30 nM ATRA for 7 days and then infected with RV for 24 h. Quantitative PCR analysis of DUOX2 mRNA expression in RV-infected cells was compared with RV-uninfected cells. Data are expressed as fold induction of DUOX2 normalized to β-actin by defining 2−ΔCt cycle threshold value from the ATRA-untreated, uninfected sample as 1.
