Fig. 2.

DEP potentiates the proinflammatory response of LPS and flagellin in epithelial cell and monocyte cocultures. Epithelial cell/monocyte cocultures were established as described in Fig. 1. Cells were stimulated with 50 μg/ml DEP in the presence or absence of 0.01 pg/ml LPS (A) or 0.5 μg/ml S. typhimurium flagellin (B). After 24 h, levels of CXCL8 in the supernatant were determined by ELISA. Data shown are means ± SE of n = 5–6 replicates. Each replicate was performed at a separate passage with freshly prepared monocytes from different donors. Significant differences in CXCL8 are indicated by ***P < 0.001 compared with DEP or LPS alone, as measured by 2-way ANOVA and Bonferroni's posttest.