Fig. 5.

LPS-induced extracellular IL-1β release is reduced in epithelial cell/monocyte cocultures stimulated in the presence of DEP. Epithelial cell/monocyte cocultures were established as described in Fig. 1. Cells were stimulated with 50 μg/ml DEP, 1 pg/ml LPS, or both DEP and LPS. After 24 h, levels of IL-1β in the supernatants were determined by ELISA. Data shown are means ± SE of n = 7 replicates. Each replicate was performed at a separate passage with freshly prepared monocytes from a different donor. Significant differences are indicated by **P < 0.01, measured by 1-way ANOVA and Tukey's posttest.