Fig. 1.

Effects of IL-6 on mRNA expression of epithelial sodium channel (ENaC) α-, β-, and γ-subunits and prostasin. M-1 cells, which were serum-deprived for 12 h, were treated with 100 ng/ml IL-6 for 12 h. At 12 h after treatment, total RNA was extracted from M-1 cells, and 5 μg of total RNA were reverse-transcribed to cDNA with oligo(dT) primers. Abundance of each mRNA was normalized for β-actin and compared with control. Values [means ± SE (n = 5)] are expressed as fold increase over control. *P < 0.05 vs. control.