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. 2010 May 5;299(2):C298–C306. doi: 10.1152/ajpcell.00118.2010

Fig. 3.

Fig. 3.

Inactivation of RhoA prevents actin polymerization in response to ACh stimulation in canine tracheal smooth muscle tissues. A: immunoblot of soluble (G, globular) and insoluble (F, filamentous) actin in fractions from extracts of unstimulated or ACh-stimulated muscle tissues treated with RhoA T19N, wild-type RhoA (RhoA WT), C3, or with no treatment (sham or no C3). B: ratios of F-actin to G-actin were determined by quantitating F- and G-actin in extracts from each muscle strip. Inhibition of RhoA activation with C3 or RhoA T19N prevented the increase in F-actin/G-actin ratio in response to ACh stimulation. *F-actin/G-actin ratio significantly lower in ACh-stimulated muscles from RhoA T19N (n = 9) or C3-treated muscles (n = 6) compared with sham-treated or no C3 treatment (P < 0.05). There were no significant differences in values for unstimulated tissues. Values are means ± SE.