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. 2010 May 5;299(2):C251–C263. doi: 10.1152/ajpcell.00091.2009

Fig. 2.

Fig. 2.

Expression of KCNN4a, KCNN4b, and KCNN4c isoform-specific transcripts in smooth muscle and epithelial cells. KCNN4a, KCNN4b, and KCNN4c-specific mRNA fragments were amplified by RT-PCR analyses as described in materials and methods. A: RT-PCR amplification of 263 and 176 bp fragments by primer set-II (see Table 1 and legend to Fig. 1A) suggest the expression of KCNN4a/b- and KCNN4c-specific transcripts in distal colon and ileal epithelial cells, respectively. Amplification of 263 bp, but not 176 bp, fragment suggests that only KCNN4a/b-specific transcripts are expressed in colonic smooth muscle cells. B: amplification of 780 and 149 bp fragments by primer set-III (see Table 1 and legend to Fig. 1A) indicate the expression of KCNN4a- and KCNN4b/c-specific transcripts in colonic smooth muscle and epithelial cells, respectively. C: amplification of 260-bp fragment by primer set-IV (see Table 1 and legend to Fig. 1A) indicates the expression of KCNN4b/c-specific transcripts in colonic epithelial but not in smooth muscle cells. D: the 263- and 176-bp fragments amplified by primer set-II are present in epithelial cells from all intestinal segments. D: the 263- and 176-bp fragments amplified by primer set-II are present in IEC-6 cells. Amplification of rat GAPDH specific segment was used as internal control (E).