Fig. 7.

Effect of TRAM-34 concentrations on DC-EBIO-enhanced ⁸⁶Rb efflux in KCNN4b (circles) and KCNNc + BK β1 (squares) cRNA-injected oocytes. ⁸⁶Rb loading and effluxes were performed as described in materials and methods. ⁸⁶Rb efflux was measured for 5 s by placing the oocytes in K⁺-free medium containing varying concentrations of TRAM-34 (0–100 μM). Hill plot analyses of the data yielded half-maximal inhibitory concentration (K_i) for TRAM-34 of ∼0.6 and 5 μM for KCNN4b- and KCNN4c-mediated ⁸⁶Rb effluxes, respectively.