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. 2010 May 5;299(2):C251–C263. doi: 10.1152/ajpcell.00091.2009

Fig. 8.

Fig. 8.

Immunofluoresence localization of KCNN4-like proteins in normal rat distal colon. The anti-KCNN4-abc antibody recognizes the COOH-terminal region of KCNN4 proteins, which is common to KCNN4a, KCNN4b, and KCNN4c isoforms, localizes KCNN4-like proteins (green) in both apical and lateral membranes of both surface, and crypt epithelial cells in rat distal colon (left). Zonula occludens-1 (ZO-1) (red) counterstains for junctional complexes of epithelial cells using an anti-ZO1 monoclonal antibody, indicating the apical most end of the lateral membrane (middle). Merge (orange) shows overlay of both proteins (left). Arrow-heads indicate positive apical staining, whereas arrows indicate positive lateral membrane staining of anti-KCNN4-abc. Zoom = ×4; scale bar is 40 μm (top) and 10 μm (bottom). Similar observations were obtained with tissues from three rats.