Table 1.

Primers used to amplify the full-length and specific fragments of KCNN4a, KCNN4b, and KCNN4c splice variants

Primer Set	Primer Sequence	Primer Location in KCNN4, (Accession No. F156554)	Isoform	Expected Size cDNA Fragment, bp
I	Sense: 5′-CCCAGGGTGAGCAGAAACA-3′	28-45	KCNN4a	2054
	Antisense 5′-GTCCTGAGTAGAATGTAGATCGA-3′	1450-1428	KCNN4b	1423
			KCNN4c	1336
II	Sense: 5′-GAGCTGGTGACTGGCCT-3′	153-168	KCNN4a	263
	Antisense 5′-CGTTGTCAGTCATGAACAGCT-3′	416-396	KCNN4b	263
			KCNN4c	176
III	Sense: 5′-CACCTTGCCCTGGAGAAGA-3′	1301-1319	KCNN4a	780
	Antisense: 5′-GTCCTGAGTAGAATGTAGATCGA-3′	1450-1428	KCNN4b	149
			KCNN4c	149
IV	Sense: 5′-CACCTTGCCCTGGAGAAGA-3′	1301-1319	KCNN4a	None
	Antisense: 5′-CCACTTGTGTCCTGGCATCT-5′	1561-1542	KCNN4b	260
			KCNN4c	260