Fig. 3.

Inhibition of DIC-mediated GSH transport increases oxidative stress in rat cortical mitochondria. A: FACS analysis of reactive oxygen species (ROS) content in cortical mitochondria using glutamate/malate as the respiration substrate. DHR-123, dihydrorhodamine-123; MFI, median fluorescence intensity. B: FACS analysis of ROS content in cortical mitochondria using succinate as the respiration substrate. C: summary of intramitochondrial ROS levels in 4 individual experiments. Increased ROS levels were evident in the M and M/GSH/BM groups only when complex I substrates glutamate/malate were used to support respiration. Data are means ± SE.