Fig. 5.
Inhibition of DIC-mediated GSH transport alters IMM functions. A: membrane potential was drastically reduced in M/GSH/BM in comparison with either M0 or M/GSH, indicating severe impairment with DIC inhibition. Shown is a representative experiment. TMRM, tetramethylrhodamine methyl ester. B: state 4 respiration rates increased in the M/GSH/BM group, especially when complex I substrates were used, indicating increased oxygen consumption under basal conditions with DIC inhibition. Data are means ± SE. C: phosphorus-to-oxygen (P/O) ratio values decreased in the M/GSH/BM group regardless of the respiration substrate used, indicating a reduced efficiency in oxidative-phosphorylation in these mitochondria with DIC inhibition. Data are means ± SE.