Fig. 6.

Inhibition of DIC-mediated GSH transport impairs complex I activity A–C: representative activity assays for complexes I, II, and III, respectively. A: decreased consumption of NADH in M/GSH/BM, indicating a reduced complex I enzymatic activity with DIC inhibition. B: similar rates in 2,6-dichlorophenol indophenol (DCIP) consumption in all three samples, indicating no difference in complex II activity. C: similar rates in accumulation of reduced cytochrome c, indicating no difference in complex III activity. D–F: summaries of complexes I, II, and III activities in multiple experiments. Only complex I activity was significantly impaired in the M/GSH/BM group in which DIC was inhibited. Data are means ± SE; n = 8 for complexes I and II and n = 5 for complex III.