Fig. 8.

DIC gene silencing decreases mGSH in PC12 cells. A: decreased DIC and OGC protein expression in PC12 cells following gene silencing with short hairpin (sh)RNA. Shown are representative Western blots from 4–5 experiments. Cytochrome c was used as a loading control. B: mitochondria from PC12 cells were incubated at 20°C for 30 min in buffer containing 2.5 mM GSH. mGSH content decreased in the DIC-shRNA group but was unaffected in the OGC-shRNA group, indicating that DIC was responsible for GSH transport in these mitochondria. These results are in line with those obtained from experiments using pharmacological agents. Data are means ± SE.