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Pyruvate decreases glycolytic enzyme levels and glucose uptake. A and B: protein content of glucose transporter 4 (GLUT4) (A) and phosphofructokinase (PFK) (B) relative to teEF2 protein content. C: 2-deoxy-[3H]d-glucose (2DG) uptake was assessed either in the basal state (control, CTL) or following 30-min insulin treatment (INS). *Significantly different from NaCl (P < 0.05); †significantly different from NaCl-insulin treatment (P < 0.05).
