Fig. 1.

Transient knockdown of protein tyrosine phosphatase, PTP-PEST, enhances colon carcinoma cell motility. A: Smart Pool small interfering (si)RNA knockdown of PTP-PEST (SP) in KM12C colon carcinoma cells leads to increased motility toward collagen I using a haptotaxis assay. Control refers to KM12C cells that have been electroporated only. NT, nontargeting siRNA control. B: siRNA knockdown of PTP-PEST in KM12C cells enhances chemotaxis toward lysophosphatidic acid (LPA), hepatocyte growth factor (HGF), or serum relative to the absence of a chemoattractant (None). C: PTP-PEST expression is efficiently decreased by the Smart Pool siRNA. Neither control nor nontargeting siRNA affects migration or PTP-PEST protein expression (n = 4). Actin was used as a loading control.