Fig. 11.

Neomycin introduces a long-lived subconductance state in cerebral artery myocyte SR channels. Unitary current records of SR native channels after bilayer reconstitution before (A) and after (B) application of 100 nM neomycin to the cis side of the bilayer. The subconductance state introduced by neomycin may last several seconds, as previously reported with cardiac RyR (53). An arrowhead on the left of each panel highlights the baseline (nonconducting channel level). In B, an arrow on the right points to a transition from the neomycin-induced subconductance to the nonconducting channel level.