Fig. 7.
Caffeine applied to the bilayer cis side increases the NPo of reconstituted SR ryanodine-sensitive channels. A: unitary current recordings of SR Ca2+- and ryanodine-sensitive channels in absence (top) and presence of 0.1 (2nd trace) or 1 mM (3rd trace) caffeine added to the cis solution. Further application of 80 μM ruthenium red (4th trace) drastically suppresses channel activity (see main text); V = +35 mV. NPo was obtained as described for Fig. 2. B: averaged NPo in response to caffeine added to the cis solution. *Different from control, P < 0.05; ***different from control, P < 0.001 (n = 8).