Table 1.

PCR primers used for detection of RyR1, RyR2, and RyR3 transcripts in cerebral artery smooth muscle, brain, heart, and skeletal muscle

Primer	Location on cDNA	Nucleotide Sequence 5′-3′	Predicted PCR Fragments Length	Reference Sequences
RyR3RLTF	13282–13303	CAGACCAAGTTACTGCACTACC (22mer, Tm = 58.6)	121 bp	XM_001080527
RYR3RLTR	13383–13403	TCTAAAGGCTCTTCCGTGACC (21mer, Tm = 58.8)
RYRRLT2F	13618–13619	GCTGAACTATTTTGCTCGCAAC (22mer, Tm = 58.4)	110 bp	EU346200
RYRRLT2R	13707–13727	CCACAGAAGAAGTGGAGACCT (21mer, Tm = 58.8)
RYRRLT1F	13641–13664	CTTGAACTACTTGTCGAGGAACTT (24mer, Tm = 58.4)	109 bp	XM_001078539
RYRRLT1R	13731–13749	TGGCGGAGAGTCTGAAACC (19mer, Tm = 58.5)