Fig. 4.

Parietal cells expressing ezrin-T567D (TD)-CFP have a distinctly different time course of appearance and distribution of the ezrin-TD mutant than either ezrin-WT or the ezrin-TA mutant. Similar to other experiments, cell cultures were infected with rAd-ezrin-TD-CFP construct and prepared for immunocytochemistry at sequential times. Early expression ezrin-TD mutant is observed primarily on apical vacuoles (green), and by 24 h there is distinct basal expression of ezrin-TD mutant; usually, in these cases, H-K-ATPase is also located to the same basal sites. Bar markers = 20 μm.