Fig. 7.

Accumulation of cytosolic and mitochondrial Ca²⁺ in cardiomyocytes exposed to oxidative stress is attenuated by partial mitochondrial depolarization. Isolated cardiomyocytes were coloaded with cytosolic (A) and mitochondrial (B) Ca²⁺ indicators, fluo-4 AM and rhod-2 AM, respectively, and their fluorescence intensities were recorded simultaneously. Upon application of H₂O₂, fluo-4 and rhod-2 fluorescence intensities increased. In cells treated with DNP (200 nM), this effect was attenuated. Summarized data are means ± SE; n = 11–17/group. *P < 0.05 vs. Ctrl.