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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: J Infect Dis. 2010 Jul 15;202(2):184–191. doi: 10.1086/653823

Figure 4.

Figure 4

JCV genome in B cells was virion protected. Infected B cells harvested at different time points after infection were mechanically lysed by repeated freeze-thaw, and untreated or treated with DNase. DNA was extracted, concentration was measured and JCV VP-1 DNA was quantitated by qPCR. (A) Total cellular DNA recovered increased from day 0 to day 15 suggesting B cells were actively replicating and DNase effectively reduced DNA concentration. (B) JCV VP-1 DNA copies decreased from days 0 to 15 after infection as expected but DNase treatment had very little effect on the viral DNA suggesting that JCV genome in the B cells was virion protected from DNase digestion.