Tissue stained for cyclin D1, treated with PAS and diastase digested (no counterstain) A–C. A. Non-tumor tissue, globules, ~2 cyclin D1 positive nuclei, arrow B. Tissue D, tumor with tiny globules, no cyclin D1 staining shown C. Cyclin D1 in tumor hepatocyte with globules, arrow. D.(No PAS stain): Ki67 stain in tumor, globules appear as white spheres. (Original magnification 400X.) E. SOCS3, Tissue D, transitional region on left, tumor on right F. & G. PSTAT3 (enhanced DAB only), transitional left, tumor right.