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. 2010 Jul 2;29(15):2553–2565. doi: 10.1038/emboj.2010.129

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Copyright © 2010, European Molecular Biology Organization

This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial No Derivative Works 3.0 Unported License, which permits distribution and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission.

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USP7 and USP11 bind to PRC1 components. (A, B) Co-precipitation of HA-USP7 (A) and HA-USP11 (B) with Flag-tagged MEL18, BMI1, CBX8, RING1 and RING2 from transiently transfected 293T cells. Input samples are shown in the left panels, and the Flag IPs in the right panels. (C) Co-precipitation of endogenous USP7 and USP11 with transiently expressed Flag-tagged MEL18, BMI1, CBX8, RING1 and RING2. (D) Co-precipitation of endogenous CBX8, BMI1, USP7 and USP11 from nuclear extracts of 293T cells using an affinity-purified CBX8 antiserum immobilised on Sepharose beads.