Figure 2.

Lpm inhibits abortive RNA synthesis at the T7A1 and lacUV5 promoters. (A) Inhibition of dinucleotide (AU) and tetranucleotide (AAUU) synthesis by 100 μM of Lpm. Transcription was initiated at the T7A1 and lacUV5 promoters. The [³²P]-RNA products separated in a 30% PAGE-7MUrea gel are shown. (B) Effect of ApA primer on the inhibition by Lpm. Transcription initiated at the lacUV5 promoter in the presence of U, G, and A or U, G, A and ApA in the presence of increasing concentrations of Lpm. The [³²P]-RNA products separated in a 24% PAGE-7MUrea gel are shown.