Figure 6.

UV cross-linking and EMSA to probe the DNA–protein interactions in the RNAP-scaffold complex in the presence of Lpm (A, B). The UV cross-linked RNAP-scaffold complexes formed without or in the presence of 100 μM of Lpm were resolved by EMSA (A) or SDS–PAGE (B). (C) UV cross-linking of the oligonucleotides used in scaffold assembly (ssDNA) and assembled scaffold to σ⁷⁰ and holoenzyme. The position of the label either on template (T) or non-template (NT) DNA strands is indicated. Cross-linked complexes resolved on SDS–PAGE are shown.