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. 2010 Jun 25;29(15):2646–2658. doi: 10.1038/emboj.2010.131

Figure 4.

Figure 4

Hh regulates stem cell self-renewal through a p53-independent pathway. (A, B) RT–qPCR (A) and western blot (B) analysis of stemness markers and Gli1 in neurospheres from p53-deficient mouse cerebella (p53−/−). (A) Results are expressed as mean values of five different neurosphere cultures from p53−/− mice with respect to p53+/+ wild type (dashed line). (C) Secondary neurosphere-forming assay of p53−/− cells with respect to cells from p53 wild-type mice. (D) Western blot analysis of Smo, Gli1 and Nanog in p53−/− neurospheres after transfection with siRNA against Smo (siSmo) compared with control siRNA (Ctrl). (E, F) Secondary neurosphere-forming assay (E) and representative bright field images (F) of cerebellar p53−/− cells after transfection with siRNA against Smo (siSmo) or control siRNA (siCtrl). *P<0.05 versus untreated p53+/+ cells (A, C) or siCtrl (E).