Figure 3.

Hdac1 and Hdac2 regulate cell cycle progression independent of p53 or p21^Cip. (A) Western blot analysis of wild-type (WT), Hdac1KO and DKO MEF protein lysates for Hdac1, Hdac2 and p53. γ-Irradiated wild-type cells expressing either control or p53 shRNA were used as a positive and negative control, respectively. Tubulin served as a loading control. (B) Western blot analysis of protein lysates for Hdac1, Hdac2, p21^Cip, p27^Kip and p16^Ink4a of MEFs with indicated genotypes infected with retroviruses expressing control shRNA (C), p21^Cip shRNA (p21) or p53 shRNA (p53). Tubulin served as a loading control. (C) Representative pictures of wild-type, Hdac1KO, Hdac2KO or DKO MEFs infected with retroviruses expressing control shRNA, p21^Cip shRNA or p53 shRNA. (D) Growth curve analysis of wild-type, Hdac1KO, Hdac2KO and DKO MEFs, expressing either control shRNA or shRNA directed against p21^Cip and p53.