Figure 5.

Hdac1 and Hdac2 collectively suppress a senescence-inducing pathway in transformed cells. (A) Western blot analysis of 4-OHT-treated MEFs with indicated genotypes, expressing Ras^V12 and p53 shRNA for Hdac1, Hdac2, p21^Cip and Ras^V12. Tubulin served as loading control. DKO MEFs served as a control for p21^Cip expression. (B) Representative pictures of oncogenic-transformed (Ras^V12;p53KD) MEF cultures with indicated genotypes in the absence (top panels) or presence (lower panels) of 4-OHT. (C) Growth curve analysis of wild-type (triangles), Hdac1KO (diamonds) and DKO (open circles) oncogenic-transformed MEFs. (D) Quantification and representative pictures of SA-β-galactosidase positive cells in cultures of wild-type and DKO oncogenic-transformed cells. Shown are average values of six different microscopic fields.