Figure 6.

Hdac1 and Hdac2 have overlapping functions in haematopoiesis. (A) Kaplan–Meier curves of pI;pC-treated MxCre⁺, MxCre⁺;Hdac1^L/L, MxCre⁺Hdac1^L/L;Hdac2^L/+ and MxCre⁺Hdac1^L/L;Hdac2^L/L mice. (B) Total bone marrow (per femur), erythrocyte and thrombocyte numbers in peripheral blood of mice with indicated genotypes. (C) Bone marrow histology of pI;pC-treated MxCre⁺, MxCre⁺;Hdac1KO, MxCre⁺;Hdac1KO;Hdac2HET and MxCre⁺;DKO mice; left panels show haematoxylin–eosin-stained paraffin tissue sections, right panels show immunohistochemistry on paraffin tissue sections using antibodies against activated caspase-3. Note the presence of mitotic figures (asterix) in megakaryocytes of MxCre⁺;Hdac1KO;Hdac2HET mice. Magnification is × 200. (D) Average megakaryocyte number in bone marrow in six different microscopic fields of three independent mice with indicated genotype. (E) Quantification of activated caspase-3 positive cells in bone marrow of indicated pI;pC-treated genotypes. Shown are average counts in three different microscopic fields in three independent mice per genotype.