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Trypsin-mediated proteolysis of apo-TraR and TraR–AAI complexes. Radiolabeled apo-TraR (1 μM) (lanes 1–7) or TraR–AAI (1 μM) (lanes 8–14) complexes were combined with trypsin at the following concentrations: 0 μM (lanes 1 and 8), 0.125 μM (lanes 2 and 9), 0.25 μM (lanes 3 and 10), 0.5 μM (lanes 4 and 11), 1 μM (lanes 5 and 12), 2 μM (lanes 6 and 13), and 4 μM (lanes 7 and 14); incubated for 30 min at room temperature; and size fractionated by SDS/PAGE.
