FIG. 2.

GP4 pepscan profiles of sera derived from LV-infected pigs. Sera collected at 44 d p.i. with LV were diluted 1:100 and used in a pepscan analysis with overlapping peptides covering GP4 of LV. OD (450 nm) values at each peptide were determined for both the test sample and a negative serum sample, and the OD for the test sample was expressed as the relative percentage of the negative control value for each peptide separately (percentage sample/negative). Signals were considered positive when the percentage sample/negative at a certain peptide was more than two times the mean percentage sample/negative over all peptides. Graph A represents a serum sample in which antibodies against peptides 14–16 were detected (core sequence GVSAAQEKISFG). Graph B represents a serum sample in which antibodies against peptides 14–16 as well as 22–23 (core sequence ITANVTDESYLY) were detected.