FIG. 2.

Evaluation of M1-specific CD8⁺ T cell expansion by using DCs (a and b) and macrophages (c and d) as APCs infected with either integrating or nonintegrating LVs expressing FLU-M1 or GFP as an unrelated antigen. (a and c) Cells were stained with PE–Cy5-labeled anti-CD8 antibody and PE-labeled HLA-A*0201 pentamer presenting the influenza matrix M1 epitope (solid columns) or control PE-labeled HLA-A*0201 pentamer presenting HIV Gag peptide (open columns). The percentage of pentamer⁺ cells was calculated within CD8⁺ T cells. (b and d) The functionality of CD8⁺ T cells was evaluated by ELISPOT for IFN-γ in the presence of M1-specific peptide (solid columns) or HIV Gag-unrelated peptide (open columns). Results are expressed as spot-forming cells (SFC) per 1 × 10⁶ cells.