FIG. 3.

Polyfunctionality of M1-specific CD8⁺ T cells. Macrophages transduced with either integrating (LV-M1) or nonintegrating LVs expressing M1 (IDLV-M1) were used as APCs in coculture experiments with autologous lymphocytes. After 9 days cells were recovered and analyzed by intracellular staining for IFN-γ, TNF-α, and IL-2, after stimulation with M1 peptide for 6 hr. Results from one representative experiment are shown. (a) The percentages of double cytokine-producing cells were calculated on CD8⁺pentamer⁺ gated T cells and are indicated within the dot plots. (b) By gating on single cytokine-producing CD8⁺pentamer⁺ T cells, the percentages of the other cytokine-producing cells were calculated and indicated in the histograms (cells producing IFN-γ, TNF-α, and IL-2 are indicated as lined, gray, and black columns, respectively).