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. 2010 Aug 13;4:36. doi: 10.3389/fnins.2010.00036

Figure 8.

Figure 8

5-HT depolarizes EGFP expressing interneurons and increase sIPSCs recorded in pyramidal cells through the activation of 5-HT2A receptors. (A) Administration of α-methyl-5-HT (30 μM) depolarizes and excites an EGFP expressing FS interneuron (left panel). (B) Administration of 5-HT (30 μM) in the presence of tropisetron (1 μM, to block 5-HT3 receptors) and CNQX (10 μM to block AMPA/kainite receptors) results in a large increase in sIPSCs. Subsequent administration of bicuculline (30 μM) blocks all spontaneous synaptic activity in the slice, thus confirming that the recorded synaptic currents represented sIPSCs. (C) Plots illustrating the effect of 5-HT (30 μM) on sIPSC frequency recorded from pyramidal cells of layer V in slices derived from wild type mice, in slices derived from wild type mice but pretreated (>10 min) with the 5-HT2A receptor antagonist MDL100,907 (1 μM) and in slices derived from 5-HT2A receptor knockout mice.