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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Cell Signal. 2010 May 11;22(9):1350–1362. doi: 10.1016/j.cellsig.2010.05.003

Fig. 3. Leptin induced canonic signalling pathways involved in the regulation of VEGF in MT.

Fig. 3

A, B and C, representative western blots from leptin induction of phosphorylated proteins in 4T1, EMT6 and MMT, respectively. D, E and F, effects of leptin and canonic signalling inhibitors on VEGF protein levels in 4T1, EMT6, and MMT, respectively. G, H and I, effects of leptin and canonic signalling inhibitors on VEGF mRNA levels in 4T1, EMT6, and MMT, respectively. MT were incubated for 24h with mouse leptin (0 or 1.2 nM) in the presence of inhibitors of JAK2/STAT3 (AG490, 30µM), ERK1/2/MAPK (PD98059, 30µM) and PI3K/AKT1 (Wortmannin, 20µM) signalling pathways. VEGF levels were determined by ELISA (pg/ml/mgprotein). VEGF mRNA levels were quantified by real-time RT-PCR. (a) p<0.05 when comparing levels of protein or mRNA of leptin-treated cells to control (basal) with or without inhibitors. Data (mean ± standard error) representative results derived from a minimum of 3 independent experiments. B: Basal, L: leptin, AG: AG490, PD: PD98059, W: Wortmannin.