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. 2010 Aug 26;5(8):e12416. doi: 10.1371/journal.pone.0012416

Figure 3. SDS-PAGE analysis of the PEG-F(ab')2 chromatography peaks.

Figure 3

P1, P2, P2′, P3, P3′, P4, P5 and P6 correspond to the different peaks recorded during the chromatography purification. KDa: KiloDalton After conjugation of F(ab')2 to the N-hydroxysuccinimidyl ester of methoxy poly(ethylene glycol) butanoic acid (NHS-PEG), the different F(ab')2 species were purified by cation exchange chromatography. This purification removed unreacted NHS-PEG and separated the different F(ab')2 species (nonpegylated, monopegylated and multipegylated F(ab')2).