Table 1. BoNT/A1 neutralization activity of crude ascite fluids from 14 mAbs in the mouse protection assay (5 estimated MLD₅₀/mouse).

Epitopic group	Antibody	Isotype	Protective titer
A	TA2	IgG1	104
	TA10	IgG1	103
	TA11	IgG1	104
	TA12	IgG1	105
	TA14	IgG1	104
B	TA4	IgG2a	104
	TA7	IgG2a	104
	TA13	IgG2a	103
	TA17	IgG2a	104
C	TA1	IgG1	≤102
	TA5	IgG1	≤102
	TA15	IgG1	≤102
D	TA9	IgG1	102
E	TA16	IgG1	103

The protective titer is the highest dilution of the ascitic fluid that completely protected against 5 estimated MLD₅₀ of BoNT/A1 in the mouse protection assay.

Underline: non-neutralizing antibodies. Three ml of 10 estimated MLD₅₀/ml BoNT/A1 were incubated with 30 µl of serial ten-fold dilutions of ascitic fluid for 30 min at room temperature. 500 µl of the mixtures were injected intraperitoneally to give final toxin challenges of approximately 5 MLD/₅₀ into each mouse of a group of 4 for each dilution.