Table 4. Experimental and predicted melting temperatures of Escherichia coli DNA at various salt concentrations.

S. No.	Genome	Na+ Conc. (M)	DNA Conc. (g/ml)	Experimental Tm (°C)	Predicted Tm (°C) #	Experimental – Predicted Tm (°C)
1.	Escherichia coli	0.015	0.000018	70.7[20]	77.9	−7.2
2.	Escherichia coli	0.016	0.00002	75.7[49]	78.3	−2.6
3.	Escherichia coli	0.0732	0.00005	85.7[12]	86.6	−0.9
4.	Escherichia coli	0.075	0.000018	83.3[20]	85.9	−2.6
5.	Escherichia coli	0.01	0.000018	68.7[20]	75.8	−7.1
6.	Escherichia coli	0.02	0.000018	73.4[20]	79.3	−5.9
7.	Escherichia coli	0.035	0.000018	77.1[20]	82.1	−5
8.	Escherichia coli	0.05	0.000018	80.0[20]	83.8	−3.8
9.	Escherichia coli	0.1	0.000018	86.5[20]	87.3	−0.8
10.	Escherichia coli	0.12	0.000018	86.0[20]	88.2	−2.2
11.	Escherichia coli	0.195	0.000018	88.7[20]	90.6	−1.9
12.	Escherichia coli	0.6	0.000018	93.9[20]	96.2	−2.3

^# Escherichia coli K-12 genome sequence (4639675 base pairs) obtained from NCBI (NC_000913) is used for these calculations.