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. 2010 Aug 26;6(8):e1001064. doi: 10.1371/journal.ppat.1001064

Figure 2. Surface expression of wild-type and mutant CCR5 variants in vitro.

Figure 2

293T cells were transfected with expression plasmids encoding smCCR5 wild-type (orange), smCCR5Δ2 (light blue), smCCR5Δ24 (magenta) and huCCR5-wt (green), along with empty expression plasmid (dark blue). Untransfected 293T cells (shaded red) serve as controls. Cells were stained with an anti-CCR5 mAb (clone 3A9; APC-conjugated), which recognizes the N-terminal region of both human and sooty mangabey CCR5 (right panel), or an isotype-matched control antibody (left panel).