Table 1.
C-terminal fragments of EP procyclins from trypanosomes isolated from fly 7–28 days after infection (Fig. 3B)
| Ion no. | Procyclin protein | No. EP repeats | Mass | Days present | Tentative assignment |
|---|---|---|---|---|---|
| 1 | EP1-2* | 25 | 5,871 | 7, 14, 21, 28 | P35-G86-EtN |
| 2 | EP1-2* | 25 | 6,083 | 7, 14, 21, 28 | P33-G86-EtN |
| 3 | EP1-2* | 25 | 6,299 | 7, 14, 21, 28 | T31-G86-EtN |
| 4 | EP1-2* | 25 | 7,903 | 14, 21, 28 | D27-G86-EtN |
| 5 | EP1-2* | 25 | 8,018 | 7, 21, 28 | D26-G86-EtN |
| 6 | EP1-2 | 25 | 8,178 | 21, 28 | S24-G86-EtN |
| 7 | EP3-3 | 25 | 8,220 | 7, 14, 21, 28 | S24-G86-EtN |
| 8 | EP3-2 | 21 | 6,898 | 7, 14, 21, 28 | D26-G76-EtN |
| 9 | EP3-2 | 21 | 7,016 | 28 | D25-G76-EtN |
| 10 | EP3-4 | 27 | 6,322 | 7, 14, 21, 28 | P35-G90-EtN |
| 11 | EP3-4 | 27 | 6,532 | 14, 28 | P33-G90-EtN |
Because of high background and impossibility of detecting N-terminal fragments in positive ion mode after mild acid hydrolysis (11), these assignments are tentative.
*
EP3-3, if present, could also contribute to this ion.